| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This rat PCX kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Podxl |
| Gene ID: |
192181 |
| Uniprot ID: |
PODXL_RAT |
| Specificity: |
This kit recognizes Rat PCX in samples. No significant cross-reactivity or interference between Rat PCX and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Glomerular epithelium cell (podocyte) (at protein level). |
| Post Translational Modifications | N- and O-linked glycosylated. Sialoglycoprotein. |
| Function | Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. |
| Protein Name | PodocalyxinPodocalyxin-Like Protein 1PcPclp-1 |
| Cellular Localisation | Apical Cell MembraneCell ProjectionMicrovillusMembrane RaftLamellipodiumFilopodiumRuffleMembraneSingle-Pass Type I Membrane ProteinIn Single Attached Epithelial Cells Is Restricted To A Preapical Pole On The Free Plasma Membrane Whereas Other Apical And Basolateral Proteins Are Not Yet PolarizedColocalizes With Nherf2 At The Apical Plasma Membrane During Epithelial PolarizationColocalizes With Nherf1 At The Trans-Golgi Network (Transiently) And At The Apical Plasma MembraneIts Association With The Membrane Raft Is TransientForms GranularPunctuated PatternForming PatchesPreferentially Adopting A Polar DistributionLocated On The Migrating Poles Of The Cell Or Forming Clusters Along The Terminal Ends Of Filipodia Establishing Contact With The Endothelial CellsColocalizes With The Submembrane Actin Of LamellipodiaParticularly Associated With RufflesColocalizes With Vinculin At Protrusions Of CellsColocalizes With Itgb1Colocalizes With Actin FilamentsEzr And Nherf1 In A Punctate Pattern At The Apical Cell Surface Where Microvilli FormColocalizes With Ezr And Nherf2 At The Apical Cell Membrane Of Glomerular Epithelium CellsColocalizes With Pard3PrkciExoc5OclnRab11a And Rab8a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Luminogenesis |
| Alternative CLIA Names | Podocalyxin CLIA kitPodocalyxin-Like Protein 1 CLIA kitPc CLIA kitPclp-1 CLIA kitPodxl CLIA kitPclp1 CLIA kit |
| Specificity | This kit recognizes Rat PCX in samples. No significant cross-reactivity or interference between Rat PCX and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-103 | 94 |
| EDTA plasma(n=8) | 101-115 | 106 |
| Cell culture media(n=8) | 91-102 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.00 | 2.87 | 7.53 | 1.10 | 3.13 | 7.94 |
| Standard deviation | 0.12 | 0.27 | 0.62 | 0.10 | 0.36 | 0.50 |
| CV (%) | 12.00 | 9.41 | 8.23 | 9.09 | 11.50 | 6.30 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
