| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.94ng/mL |
| Detection Limit: |
1.56~100ng/mL |
| Short Description: |
This Oga Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Oga |
| Gene ID: |
154968 |
| Uniprot ID: |
OGA_RAT |
| Specificity: |
This kit recognizes Rat NAGase in samples. No significant cross-reactivity or interference between Rat NAGase and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other.cleavage does not decrease enzyme activity. |
| Function | Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 2: Lacks enzyme activity. Isoform 3: Lacks enzyme activity. |
| Protein Name | Protein O-GlcnacaseOgaBeta-N-AcetylhexosaminidaseBeta-HexosaminidaseBifunctional Protein NcoatMeningioma-Expressed Antigen 5N-Acetyl-Beta-D-GlucosaminidaseN-Acetyl-Beta-Glucosaminidase |
| Cellular Localisation | NucleusCytoplasm |
| Alternative ELISA Names | Protein O-Glcnacase ELISA kitOga ELISA kitBeta-N-Acetylhexosaminidase ELISA kitBeta-Hexosaminidase ELISA kitBifunctional Protein Ncoat ELISA kitMeningioma-Expressed Antigen 5 ELISA kitN-Acetyl-Beta-D-Glucosaminidase ELISA kitN-Acetyl-Beta-Glucosaminidase ELISA kitOga ELISA kitHexc ELISA kitMea5 ELISA kitMgea5 ELISA kit |
| Specificity | This kit recognizes Rat NAGase in samples. No significant cross-reactivity or interference between Rat NAGase and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-106 | 99 |
| EDTA plasma (n=8) | 90-105 | 98 |
| Cell culture media (n=8) | 87-101 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 4.80 | 15.43 | 40.18 | 4.71 | 14.04 | 39.06 |
| Standard deviation | 0.30 | 0.82 | 1.99 | 0.28 | 0.57 | 1.20 |
| CV (%) | 6.25 | 5.31 | 4.95 | 5.94 | 4.06 | 3.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
