| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This Nos3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Nos3 |
| Gene ID: |
24600 |
| Uniprot ID: |
NOS3_RAT |
| Specificity: |
This kit recognizes Rat NOS3/eNOS in samples. No significant cross-reactivity or interference between Rat NOS3/eNOS and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Phosphorylation by AMPK at Ser-1176 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-494, resulting in inhibition of activity. |
| Function | Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. |
| Protein Name | Nitric Oxide Synthase 3Constitutive NosCnosEc-NosNos Type IiiNosiiiNitric Oxide Synthase - EndothelialEndothelial NosEnos |
| Database Links | Reactome: R-RNO-1222556Reactome: -RNO-1474151Reactome: -RNO-203615Reactome: -RNO-203641Reactome: -RNO-203754Reactome: -RNO-392154Reactome: -RNO-5218920Reactome: -RNO-9009391 |
| Cellular Localisation | MembraneCaveolaCytoplasmCytoskeletonGolgi ApparatusCell MembraneSpecifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell CycleWhich Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity |
| Alternative ELISA Names | Nitric Oxide Synthase 3 ELISA kitConstitutive Nos ELISA kitCnos ELISA kitEc-Nos ELISA kitNos Type Iii ELISA kitNosiii ELISA kitNitric Oxide Synthase - Endothelial ELISA kitEndothelial Nos ELISA kitEnos ELISA kitNos3 ELISA kit |
| Specificity | This kit recognizes Rat NOS3/eNOS in samples. No significant cross-reactivity or interference between Rat NOS3/eNOS and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-103 | 95 |
| EDTA plasma (n=8) | 86-102 | 93 |
| Cell culture media (n=8) | 95-106 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 48.40 | 111.20 | 492.00 | 53.00 | 121.10 | 496.00 |
| Standard deviation | 3.00 | 6.70 | 25.10 | 2.70 | 5.20 | 17.90 |
| CV (%) | 6.20 | 6.03 | 5.10 | 5.09 | 4.29 | 3.61 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
