| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.115ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This NHE3 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Slc9a3 |
| Gene ID: |
24784 |
| Uniprot ID: |
SL9A3_RAT |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Most abundant in colon and small intestine, followed by kidney and stomach. In kidney, expressed in proximal tubules and outer medulla (at protein level). |
| Post Translational Modifications | Phosphorylated by PRKACA at Ser-552 and Ser-605, which inhibits activity. Phosphorylation of Ser-605 is essential for cAMP-mediated inhibition of SLC9A3. Phosphorylation at Ser-661 by SGK1 is associated with increased abundance at the cell membrane. Phosphorylation at Ser-716 by CSNK2A1 regulates SLC9A3 activity through the formation of multiple signaling complexes. |
| Function | Plasma membrane Na(+)/H(+) antiporter. Exchanges intracellular H(+) ions for extracellular Na(+) in 1:1 stoichiometry, playing a key role in salt and fluid absorption and pH homeostasis. Major apical Na(+)/H(+) exchanger in kidney and intestine playing an important role in renal and intestine Na(+) absorption and blood pressure regulation. |
| Protein Name | Sodium/Hydrogen Exchanger 3Na(+/H(+ Exchanger 3Nhe-3Solute Carrier Family 9 Member 3 |
| Database Links | Reactome: R-RNO-425986 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCell MembraneRecycling Endosome MembraneEarly Endosome MembraneIn Intestinal Epithelial CellsLocalizes To The Ileal Brush BorderAngiotensin-2 Enhances Apical ExpressionTranslocates Between Intracellular Compartments And The Plasma Membrane Via A Clathrin-Mediated Pathway |
| Alternative ELISA Names | Sodium/Hydrogen Exchanger 3 ELISA kitNa(+/H(+ Exchanger 3 ELISA kitNhe-3 ELISA kitSolute Carrier Family 9 Member 3 ELISA kitSlc9a3 ELISA kitNhe3 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
