| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.055ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This NGFIB Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Nr4a1 |
| Gene ID: |
79240 |
| Uniprot ID: |
NR4A1_RAT |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in lung, brain and superior cervical ganglia. High levels are seen in the adrenal tissue. |
| Post Translational Modifications | Phosphorylated at Ser-350 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli. Phosphorylation of Ser-350 results in decrease in NBRE binding while phosphorylation of Ser-340 has little effect on it. Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1. |
| Function | Orphan nuclear receptor. Binds the NGFI-B response element (NBRE) 5'-AAAGGTCA-3'. Binds 9-cis-retinoic acid outside of its ligand-binding (NR LBD) domain. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Regulates the inflammatory response in macrophages by regulating metabolic adaptations during inflammation, including repressing the transcription of genes involved in the citric acid cycle (TCA). Inhibits NF-kappa-B signaling by binding to low-affinity NF-kappa-B binding sites, such as at the IL2 promoter. May act concomitantly with NR4A2 in regulating the expression of delayed-early genes during liver regeneration. Plays a role in the vascular response to injury. In the cytosol, upon its detection of both bacterial lipopolysaccharide (LPS) and NBRE-containing mitochondrial DNA released by GSDMD pores during pyroptosis, it promotes non-canonical NLRP3 inflammasome activation by stimulating association of NLRP3 and NEK7. |
| Protein Name | Nuclear Receptor Subfamily 4 Group A Member 1Nur77Nerve Growth Factor-Induced Protein I-BNgfi-BOrphan Nuclear Receptor Hmr |
| Database Links | Reactome: R-RNO-198693Reactome: -RNO-383280 |
| Cellular Localisation | NucleusCytoplasmCytosolMitochondrionNuclear Export To The Cytosol Is Xpo1-Mediated And Positively Regulated By Ifi27Translocation To The Mitochondrion Upon Interaction With Rxra And Upon The Presence Of 9-Cis Retinoic Acid |
| Alternative ELISA Names | Nuclear Receptor Subfamily 4 Group A Member 1 ELISA kitNur77 ELISA kitNerve Growth Factor-Induced Protein I-B ELISA kitNgfi-B ELISA kitOrphan Nuclear Receptor Hmr ELISA kitNr4a1 ELISA kitHmr ELISA kitNgfib ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
