• Rat NGF (Nerve Growth Factor) CLIA Kit (STJC0001814)

Rat NGF (Nerve Growth Factor) CLIA Kit (STJC0001814)

SKU:
STJC0001814

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This rat NGF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Ngf
Gene ID: 310738
Uniprot ID: NGF_RAT
Specificity: This kit recognizes Rat NGF in samples. No significant cross-reactivity or interference between Rat NGF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Detected in the granule and pyramidal cell layer in the hippocampus.
Function Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form.
Protein Name Beta-Nerve Growth Factor
Beta-Ngf
Database Links Reactome: R-RNO-167060
Reactome: -RNO-170968
Reactome: -RNO-170984
Reactome: -RNO-177504
Reactome: -RNO-187042
Reactome: -RNO-198203
Reactome: -RNO-205017
Reactome: -RNO-205025
Reactome: -RNO-205043
Reactome: -RNO-209543
Reactome: -RNO-209560
Reactome: -RNO-209563
Cellular Localisation Secreted
Endosome Lumen
Prongf Is Endocytosed After Binding To The Cell Surface Receptor Formed By Sort1 And Ngfr
Alternative CLIA Names Beta-Nerve Growth Factor CLIA kit
Beta-Ngf CLIA kit
Ngf CLIA kit
Ngfb CLIA kit
Specificity This kit recognizes Rat NGF in samples. No significant cross-reactivity or interference between Rat NGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 93
EDTA plasma(n=8) 96-111 104
Cell culture media(n=8) 100-113 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 43.32 103.86 292.54 46.68 97.66 265.52
Standard deviation 4.82 8.87 18.17 4.73 8.50 28.70
CV (%) 11.13 8.54 6.21 10.13 8.70 10.81
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance