| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
23.44pg/mL |
| Detection Limit: |
39.06~2500pg/mL |
| Short Description: |
This Ngf Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Ngf |
| Gene ID: |
310738 |
| Uniprot ID: |
NGF_RAT |
| Specificity: |
This kit recognizes Rat NGF in samples. No significant cross-reactivity or interference between Rat NGF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form. |
| Protein Name | Beta-Nerve Growth FactorBeta-Ngf |
| Database Links | Reactome: R-RNO-167060Reactome: -RNO-170968Reactome: -RNO-170984Reactome: -RNO-177504Reactome: -RNO-187042Reactome: -RNO-198203Reactome: -RNO-205017Reactome: -RNO-205025Reactome: -RNO-205043Reactome: -RNO-209543Reactome: -RNO-209560Reactome: -RNO-209563 |
| Cellular Localisation | SecretedEndosome LumenProngf Is Endocytosed After Binding To The Cell Surface Receptor Formed By Sort1 And Ngfr |
| Alternative ELISA Names | Beta-Nerve Growth Factor ELISA kitBeta-Ngf ELISA kitNgf ELISA kitNgfb ELISA kit |
| Specificity | This kit recognizes Rat NGF in samples. No significant cross-reactivity or interference between Rat NGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-107 | 99 |
| EDTA plasma (n=8) | 93-108 | 99 |
| Cell culture media (n=8) | 90-107 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 133.12 | 309.15 | 1103.70 | 127.82 | 305.03 | 1172.82 |
| Standard deviation | 8.52 | 17.78 | 53.86 | 6.62 | 12.57 | 56.76 |
| CV (%) | 6.40 | 5.75 | 4.88 | 5.18 | 4.12 | 4.84 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
