• Rat Nefl (Neurofilament light polypeptide) Sandwich ELISA (STJE0006478)
  • Rat Nefl (Neurofilament light polypeptide) Sandwich ELISA (STJE0006478)

Rat Nefl (Neurofilament light polypeptide) Sandwich ELISA (STJE0006478)

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STJE0006478

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Nefl Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Nefl
Gene ID: 83613
Uniprot ID: NFL_RAT
Specificity: This kit recognizes Rat NEFL in samples. No significant cross-reactivity or interference between Rat NEFL and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O-glycosylated.contains three N-acetylglucosamine side chains. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Light Polypeptide
Nf-L
68 Kda Neurofilament Protein
Neurofilament Triplet L Protein
Database Links Reactome: R-RNO-438066
Reactome: -RNO-5673001
Cellular Localisation Cell Projection
Axon
Cytoplasm
Cytoskeleton
Alternative ELISA Names Neurofilament Light Polypeptide ELISA kit
Nf-L ELISA kit
68 Kda Neurofilament Protein ELISA kit
Neurofilament Triplet L Protein ELISA kit
Nefl ELISA kit
Nf68 ELISA kit
Nfl ELISA kit
Specificity This kit recognizes Rat NEFL in samples. No significant cross-reactivity or interference between Rat NEFL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 94
EDTA plasma (n=8) 87-100 93
Cell culture media (n=8) 88-100 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 55.40 114.37 334.38 60.53 105.61 345.32
Standard deviation 3.86 6.23 12.20 3.32 4.65 11.43
CV (%) 6.97 5.45 3.65 5.48 4.40 3.31
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance