Applications: |
CLIA |
Reactivity: |
Rat |
Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Sensitivity: |
18.75pg/mL |
Detection Limit: |
31.25~2000pg/mL |
Short Description: |
This rat MBP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
Assay Time: |
3.5h |
Detection: |
Chemiluminescence |
Gene Symbol: |
Mbp |
Gene ID: |
24547 |
Uniprot ID: |
MBP_RAT |
Specificity: |
This kit recognizes Rat MBP in samples. No significant cross-reactivity or interference between Rat MBP and analogues was observed. |
Sample Type: |
Serum, plasma and other biological fluids |
Tissue Specificity | Found in both the central and the peripheral nervous system. |
Post Translational Modifications | As in other animals, several charge isomers may be produced as a result of optional post-translational modifications, such as phosphorylation of serine or threonine residues, deamidation of glutamine or asparagine residues, citrullination and methylation of arginine residues. Arg-131 was found to be 44% monomethylated and 11% symmetrically dimethylated. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells. |
Function | Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane. |
Protein Name | Myelin Basic ProteinMbp |
Cellular Localisation | Myelin MembranePeripheral Membrane ProteinCytoplasmic SideCytoplasmic Side Of Myelin |
Alternative CLIA Names | Myelin Basic Protein CLIA kitMbp CLIA kitMbp CLIA kit |
Specificity | This kit recognizes Rat MBP in samples. No significant cross-reactivity or interference between Rat MBP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 91-103 | 98 |
EDTA plasma(n=8) | 92-103 | 97 |
Cell culture media(n=8) | 95-107 | 100 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 106.37 | 311.90 | 882.94 | 97.41 | 326.70 | 836.00 |
Standard deviation | 11.13 | 31.38 | 57.30 | 9.65 | 29.14 | 59.61 |
CV (%) | 10.46 | 10.06 | 6.49 | 9.91 | 8.92 | 7.13 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance