| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This Lgals3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Lgals3 |
| Gene ID: |
83781 |
| Uniprot ID: |
LEG3_RAT |
| Specificity: |
This kit recognizes Rat GAL3 in samples. No significant cross-reactivity or interference between Rat GAL3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Protein Name | Galectin-3Gal-335 Kda LectinCarbohydrate-Binding Protein 35Cbp 35Galactose-Specific Lectin 3Ige-Binding ProteinLaminin-Binding ProteinLectin L-29Mac-2 Antigen |
| Database Links | Reactome: R-RNO-6798695 |
| Cellular Localisation | CytoplasmNucleusSecretedSecreted By A Non-Classical Secretory Pathway And Associates With The Cell SurfaceCan Be SecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative ELISA Names | Galectin-3 ELISA kitGal-3 ELISA kit35 Kda Lectin ELISA kitCarbohydrate-Binding Protein 35 ELISA kitCbp 35 ELISA kitGalactose-Specific Lectin 3 ELISA kitIge-Binding Protein ELISA kitLaminin-Binding Protein ELISA kitLectin L-29 ELISA kitMac-2 Antigen ELISA kitLgals3 ELISA kit |
| Specificity | This kit recognizes Rat GAL3 in samples. No significant cross-reactivity or interference between Rat GAL3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-104 | 97 |
| EDTA plasma (n=8) | 90-105 | 98 |
| Cell culture media (n=8) | 90-106 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 97.98 | 274.43 | 937.93 | 105.57 | 286.75 | 924.15 |
| Standard deviation | 5.58 | 12.71 | 37.70 | 5.50 | 16.03 | 37.71 |
| CV (%) | 5.70 | 4.63 | 4.02 | 5.21 | 5.59 | 4.08 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
