• Rat Lbp (Lipopolysaccharide-binding protein) Sandwich ELISA (STJE0005880)

Rat Lbp (Lipopolysaccharide-binding protein) Sandwich ELISA (STJE0005880)

SKU:
STJE0005880

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.13ng/mL
Detection Limit: 1.88~120ng/mL
Short Description: This Lbp Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: LbpUniprot ID=LBP_RAT"
Specificity: This kit recognizes Rat LBP in samples. No significant cross-reactivity or interference between Rat LBP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
Protein Name Lipopolysaccharide-Binding Protein
Lbp
Cellular Localisation Secreted
Cytoplasmic Granule Membrane
Membrane-Associated In Polymorphonuclear Leukocytes (Pmn) Granules
Alternative ELISA Names Lipopolysaccharide-Binding Protein ELISA kit
Lbp ELISA kit
Lbp ELISA kit
Specificity This kit recognizes Rat LBP in samples. No significant cross-reactivity or interference between Rat LBP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 94
EDTA plasma (n=8) 91-106 99
Cell culture media (n=8) 91-104 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 6.15 17.16 56.61 6.59 18.22 58.90
Standard deviation 0.32 0.98 2.94 0.39 1.04 2.93
CV (%) 5.20 5.71 5.19 5.92 5.71 4.97
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance