| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
11.8pg/mL |
| Detection Limit: |
31.2-2000pg/mL |
| Short Description: |
This KNG1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Tissue Specificity | Plasma. |
| Post Translational Modifications | Bradykinin is released from kininogen by plasma kallikrein. Phosphorylated by FAM20C in the extracellular medium. Bradykinin: Bradykinin is inactivated by ACE, which removes the dipeptide Arg-Phe from its C-terminus. |
| Function | Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII.HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin: The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action). |
| Protein Name | Kininogen-1 Cleaved Into - Kininogen-1 Heavy Chain - Bradykinin - Kininogen-1 Light Chain |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-140837Reactome: -RNO-375276Reactome: -RNO-381426Reactome: -RNO-416476Reactome: -RNO-418594Reactome: -RNO-8957275 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative ELISA Names | Kininogen-1 Cleaved Into - Kininogen-1 Heavy Chain - Bradykinin - Kininogen-1 Light Chain ELISA kitKng1 ELISA kitKng ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
