| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.064ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This KISS1R Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Kiss1r |
| Gene ID: |
78976 |
| Uniprot ID: |
KISSR_RAT |
| Sample Type: |
tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | Highest expression levels in the cerebrum and cecum. Moderate expression in the ovary, colon and placenta. Low levels in the uterus, small intestine, and thymus. Expressed only moderately in the placenta. No expression in kidney tissues. Has a complex and abundant central nervous system expression pattern. Expressed in brain regions such as pons, midbrain, thalamus, hypothalamus, hippocampus, amygdala, cortex, frontal cortex, and striatum. No expression in the cerebellum. Persistent expression is detected in hypothalamus throughout postnatal development, with maximum expression levels at puberty in both male and female. Hypothalamic expression changed throughout the estrus cycle and is significantly increased after gonadectomy, a rise that is prevented by sex steroid replacement both in males and females. |
| Post Translational Modifications | |
| Function | Receptor for metastin, a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins. |
| Protein Name | Kiss-1 ReceptorKiss-1rG-Protein Coupled Receptor 54G-Protein Coupled Receptor Ot7t175Rot7t175Kisspeptins ReceptorMetastin Receptor |
| Database Links | Reactome: R-RNO-375276Reactome: -RNO-416476 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative ELISA Names | Kiss-1 Receptor ELISA kitKiss-1r ELISA kitG-Protein Coupled Receptor 54 ELISA kitG-Protein Coupled Receptor Ot7t175 ELISA kitRot7t175 ELISA kitKisspeptins Receptor ELISA kitMetastin Receptor ELISA kitKiss1r ELISA kitGpr54 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
