| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
46.88pg/mL |
| Detection Limit: |
78.13~5000pg/mL |
| Short Description: |
This Kiss1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Kiss1 |
| Gene ID: |
289023 |
| Uniprot ID: |
KISS1_RAT |
| Specificity: |
This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals. |
| Protein Name | Metastasis-Suppressor Kiss-1Kisspeptin-1 Cleaved Into - Metastin - Kisspeptin-10Metastin45-54 |
| Database Links | Reactome: R-RNO-375276Reactome: -RNO-416476 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Metastasis-Suppressor Kiss-1 ELISA kitKisspeptin-1 Cleaved Into - Metastin - Kisspeptin-10 ELISA kitMetastin45-54 ELISA kitKiss1 ELISA kit |
| Specificity | This kit recognizes Rat KISS1 in samples. No significant cross-reactivity or interference between Rat KISS1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 92 |
| EDTA plasma (n=8) | 90-103 | 96 |
| Cell culture media (n=8) | 87-101 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 228.82 | 578.41 | 2346.06 | 244.62 | 578.38 | 2521.30 |
| Standard deviation | 13.41 | 32.04 | 79.30 | 15.78 | 27.94 | 92.28 |
| CV (%) | 5.86 | 5.54 | 3.38 | 6.45 | 4.83 | 3.66 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
