• Rat HSPA5 (Heat Shock 70kDa Protein 5) Sandwich ELISA Kit (STJE0009567)

Rat HSPA5 (Heat Shock 70kDa Protein 5) Sandwich ELISA Kit (STJE0009567)

SKU:
STJE0009567

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 0.27ng/mL
Detection Limit: 0.781-50ng/mL
Short Description: This HSPA5 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Hspa5
Gene ID: 25617
Uniprot ID: BIP_RAT
Sample Type: tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Tissue Specificity
Post Translational Modifications In unstressed cells, AMPylation at Thr-518 by FICD inactivates the chaperome activity: AMPylated form is locked in a relatively inert state and only weakly stimulated by J domain-containing proteins. In response to endoplasmic reticulum stress, de-AMPylation by the same protein, FICD, restores the chaperone activity.
Function Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation.
Protein Name Endoplasmic Reticulum Chaperone Bip
78 Kda Glucose-Regulated Protein
Grp-78
Binding-Immunoglobulin Protein
Bip
Heat Shock Protein 70 Family Protein 5
Hsp70 Family Protein 5
Heat Shock Protein Family A Member 5
Immunoglobulin Heavy Chain-Binding Protein
Steroidogenesis-Activator Polypeptide
Database Links Reactome: R-RNO-3371453
Reactome: -RNO-983170
Cellular Localisation Endoplasmic Reticulum Lumen
Melanosome
Cytoplasm
Cell Surface
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Localizes To The Cell Surface In Epithelial Cells
High Levels Of Free Iron Promotes Cell Surface Localization
Alternative ELISA Names Endoplasmic Reticulum Chaperone Bip ELISA kit
78 Kda Glucose-Regulated Protein ELISA kit
Grp-78 ELISA kit
Binding-Immunoglobulin Protein ELISA kit
Bip ELISA kit
Heat Shock Protein 70 Family Protein 5 ELISA kit
Hsp70 Family Protein 5 ELISA kit
Heat Shock Protein Family A Member 5 ELISA kit
Immunoglobulin Heavy Chain-Binding Protein ELISA kit
Steroidogenesis-Activator Polypeptide ELISA kit
Hspa5 ELISA kit
Grp78 ELISA kit
output

Information sourced from Uniprot.org

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