| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat HSPA4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Hspa4 |
| Gene ID: |
266759 |
| Uniprot ID: |
HSP74_RAT |
| Specificity: |
This kit recognizes Rat HSPA4 in samples. No significant cross-reactivity or interference between Rat HSPA4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Ubiquitous. Highly expressed in testis. |
| Protein Name | Heat Shock 70 Kda Protein 4Ischemia Responsive 94 Kda Protein |
| Database Links | Reactome: R-RNO-3371453 |
| Cellular Localisation | Cytoplasm |
| Alternative CLIA Names | Heat Shock 70 Kda Protein 4 CLIA kitIschemia Responsive 94 Kda Protein CLIA kitHspa4 CLIA kitIrp94 CLIA kit |
| Specificity | This kit recognizes Rat HSPA4 in samples. No significant cross-reactivity or interference between Rat HSPA4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 85-100 | 92 |
| EDTA plasma(n=8) | 98-113 | 106 |
| Cell culture media(n=8) | 85-96 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 95.14 | 292.09 | 763.89 | 99.11 | 314.04 | 708.54 |
| Standard deviation | 11.45 | 28.74 | 85.02 | 9.48 | 26.29 | 62.14 |
| CV (%) | 12.03 | 9.84 | 11.13 | 9.57 | 8.37 | 8.77 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
