| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.063ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This HSD3b1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Hsd3b1 |
| Gene ID: |
360348 |
| Uniprot ID: |
3BHS1_RAT |
| Sample Type: |
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
| Tissue Specificity | Adrenal glands, kidney, testes and ovaries. |
| Post Translational Modifications | |
| Function | A bifunctional enzyme responsible for the oxidation and isomerization of 3beta-hydroxy-Delta(5)-steroid precursors to 3-oxo-Delta(4)-steroids, an essential step in steroid hormone biosynthesis. Specifically catalyzes the conversion of pregnenolone to progesterone, dehydroepiandrosterone (DHEA) to 4-androstenedione, and androstenediol to testosterone. Additionally, catalyzes the interconversion between 3beta-hydroxy and 3-oxo-5alpha-androstane steroids controlling the bioavalability of the active forms. Specifically converts dihydrotestosterone to its inactive form 5alpha-androstanediol, that does not bind androgen receptor/AR. Also converts androstanedione, a precursor of testosterone and estrone, to epiandrosterone. Expected to use NAD(+) as preferred electron donor for the 3beta-hydroxy-steroid dehydrogenase activity and NADPH for the 3-ketosteroid reductase activity (Probable). |
| Protein Name | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 13 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I3-Beta-Hsd I3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase3-Beta-Hydroxysteroid 3-DehydrogenaseDelta-5-3-Ketosteroid IsomeraseDihydrotestosterone OxidoreductaseSteroid Delta-Isomerase |
| Database Links | Reactome: R-RNO-193048Reactome: -RNO-193993Reactome: -RNO-194002 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane ProteinMitochondrion Membrane |
| Alternative ELISA Names | 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 1 ELISA kit3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type I ELISA kit3-Beta-Hsd I ELISA kit3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase ELISA kit3-Beta-Hydroxy-Delta(5-Steroid Dehydrogenase ELISA kit3-Beta-Hydroxysteroid 3-Dehydrogenase ELISA kitDelta-5-3-Ketosteroid Isomerase ELISA kitDihydrotestosterone Oxidoreductase ELISA kitSteroid Delta-Isomerase ELISA kitHsd3b1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
