| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This rat HP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Hp |
| Gene ID: |
24464 |
| Uniprot ID: |
HPT_RAT |
| Specificity: |
This kit recognizes Rat HP in samples. No significant cross-reactivity or interference between Rat HP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed by the liver and secreted in plasma. |
| Function | As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. |
| Protein Name | HaptoglobinLiver Regeneration-Related Protein Lrrg173 Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain |
| Database Links | Reactome: R-RNO-2168880Reactome: -RNO-6798695 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Haptoglobin CLIA kitLiver Regeneration-Related Protein Lrrg173 Cleaved Into - Haptoglobin Alpha Chain - Haptoglobin Beta Chain CLIA kitHp CLIA kitBa1-647 CLIA kit |
| Specificity | This kit recognizes Rat HP in samples. No significant cross-reactivity or interference between Rat HP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 99 |
| EDTA plasma(n=8) | 89-105 | 97 |
| Cell culture media(n=8) | 100-116 | 106 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.85 | 19.79 | 70.89 | 11.75 | 19.97 | 76.67 |
| Standard deviation | 1.03 | 2.33 | 4.91 | 1.51 | 1.59 | 7.11 |
| CV (%) | 9.49 | 11.77 | 6.93 | 12.85 | 7.96 | 9.27 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
