• Rat HIF2a (Hypoxia Inducible Factor 2 Alpha) Sandwich ELISA Kit (STJE0009460)

Rat HIF2a (Hypoxia Inducible Factor 2 Alpha) Sandwich ELISA Kit (STJE0009460)

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STJE0009460

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 25pg/mL
Detection Limit: 78.1-5000pg/mL
Short Description: This HIF2a Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: Epas1
Gene ID: 29452
Uniprot ID: EPAS1_RAT
Sample Type: tissue homogenates, cell lysates or other biological fluids.
Tissue Specificity
Post Translational Modifications In normoxia, is probably hydroxylated on Pro-405 and Pro-530 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-851 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
Function Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT.heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Protein Name Endothelial Pas Domain-Containing Protein 1
Epas-1
Hypoxia-Inducible Factor 2-Alpha
Hif-2-Alpha
Hif2-Alpha
Database Links Reactome: R-RNO-1234158
Reactome: -RNO-1234174
Reactome: -RNO-1234176
Reactome: -RNO-8951664
Cellular Localisation Nucleus
Nucleus Speckle
Colocalizes With Hif3a In The Nucleus And Speckles
Alternative ELISA Names Endothelial Pas Domain-Containing Protein 1 ELISA kit
Epas-1 ELISA kit
Hypoxia-Inducible Factor 2-Alpha ELISA kit
Hif-2-Alpha ELISA kit
Hif2-Alpha ELISA kit
Epas1 ELISA kit
Hif2a ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance