• Rat HGF (Hepatocyte Growth Factor) CLIA Kit (STJC0001728)

Rat HGF (Hepatocyte Growth Factor) CLIA Kit (STJC0001728)

SKU:
STJC0001728

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This rat HGF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Hgf
Gene ID: 24446
Uniprot ID: HGF_RAT
Specificity: This kit recognizes Rat HGF in samples. No significant cross-reactivity or interference between Rat HGF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.
Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation.
Protein Name Hepatocyte Growth Factor
Hepatopoietin-A
Scatter Factor
Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain
Database Links Reactome: R-RNO-114608
Reactome: -RNO-1257604
Reactome: -RNO-5673001
Reactome: -RNO-6806942
Reactome: -RNO-6807004
Reactome: -RNO-6811558
Reactome: -RNO-8851805
Reactome: -RNO-8851907
Reactome: -RNO-8865999
Reactome: -RNO-8874081
Reactome: -RNO-8875513
Reactome: -RNO-8875555
Reactome: -RNO-8875656
Reactome: -RNO-8875791
Reactome: -RNO-9734091
Alternative CLIA Names Hepatocyte Growth Factor CLIA kit
Hepatopoietin-A CLIA kit
Scatter Factor CLIA kit
Sf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain CLIA kit
Hgf CLIA kit
Specificity This kit recognizes Rat HGF in samples. No significant cross-reactivity or interference between Rat HGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-112 106
EDTA plasma(n=8) 88-100 93
Cell culture media(n=8) 95-110 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 46.26 134.30 346.20 49.13 135.85 334.22
Standard deviation 4.23 14.41 24.65 6.24 12.97 23.23
CV (%) 9.14 10.73 7.12 12.70 9.55 6.95
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance