| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat GR kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Nr3c1 |
| Gene ID: |
24413 |
| Uniprot ID: |
GCR_RAT |
| Specificity: |
This kit recognizes Rat GR in samples. No significant cross-reactivity or interference between Rat GR and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Acetylation by CLOCK reduces its binding to glucocorticoid response elements and its transcriptional activity. Increased proteasome-mediated degradation in response to glucocorticoids. Phosphorylated in the absence of hormone.becomes hyperphosphorylated in the presence of glucocorticoids. The Ser-224, Ser-246 and Ser-424-phosphorylated forms are mainly cytoplasmic, and the Ser-232-phosphorylated form is nuclear. Phosphorylation at Ser-232 increases transcriptional activity. Phosphorylation at Ser-224, Ser-246 and Ser-424 decreases signaling capacity. Phosphorylation at Ser-424 may protect from glucocorticoid-induced apoptosis. Phosphorylation at Ser-224 and Ser-232 is not required in regulation of chromosome segregation. May be dephosphorylated by PPP5C, attenuates NR3C1 action. Sumoylation at Lys-297 and Lys-313 negatively regulates its transcriptional activity. Sumoylation at Lys-721 positively regulates its transcriptional activity in the presence of RWDD3. Sumoylation at Lys-297 and Lys-313 is dispensable whereas sumoylation at Lys-721 is critical for the stimulatory effect of RWDD3 on its transcriptional activity. Heat shock increases sumoylation in a RWDD3-dependent manner. Ubiquitinated.restricts glucocorticoid-mediated transcriptional signaling. |
| Function | Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling. Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. In response to induction by transcription factor EGR1/NGFI-A in the hippocampus, may play a role in the behavioral and hypothalamic-pituitary-adrenal responses to stress in offspring. Isoform A: Has transcriptional activation and repression activity. Mediates glucocorticoid-induced apoptosis. Promotes accurate chromosome segregation during mitosis. May act as a tumor suppressor. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic gene expression. |
| Protein Name | Glucocorticoid ReceptorGrNuclear Receptor Subfamily 3 Group C Member 1 |
| Database Links | Reactome: R-RNO-3371497Reactome: -RNO-383280Reactome: -RNO-4090294 |
| Cellular Localisation | Isoform A: CytoplasmNucleusMitochondrionCytoplasmCytoskeletonSpindleMicrotubule Organizing CenterCentrosomeAfter Ligand ActivationTranslocates From The Cytoplasm To The NucleusIn The Presence Of Nr1d1 Shows A Time-Dependent Subcellular LocalizationLocalizing To The Cytoplasm At Zt8 And To The Nucleus At Zt20Lacks This Diurnal Pattern Of Localization In The Absence Of Nr1d1Localizing To Both Nucleus And The Cytoplasm At Zt8 And Zt20 |
| Alternative CLIA Names | Glucocorticoid Receptor CLIA kitGr CLIA kitNuclear Receptor Subfamily 3 Group C Member 1 CLIA kitNr3c1 CLIA kitGrl CLIA kit |
| Specificity | This kit recognizes Rat GR in samples. No significant cross-reactivity or interference between Rat GR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-105 | 96 |
| EDTA plasma(n=8) | 86-98 | 92 |
| Cell culture media(n=8) | 88-101 | 93 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 98.91 | 196.48 | 690.66 | 106.70 | 200.99 | 735.86 |
| Standard deviation | 9.06 | 20.96 | 77.84 | 10.26 | 15.72 | 75.13 |
| CV (%) | 9.16 | 10.67 | 11.27 | 9.62 | 7.82 | 10.21 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
