• Rat Gast (Gastrin) Competitive ELISA (STJE0005791)

Rat Gast (Gastrin) Competitive ELISA (STJE0005791)

SKU:
STJE0005791

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This Gast Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: Gast
Gene ID: 25320
Uniprot ID: GAST_RAT
Specificity: This kit recognizes Rat GT in samples. No significant cross-reactivity or interference between Rat GT and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
Protein Name Gastrin Cleaved Into - Big Gastrin
Gastrin-34
G34 - Gastrin
Database Links Reactome: R-RNO-416476
Reactome: -RNO-881907
Cellular Localisation Secreted
Alternative ELISA Names Gastrin Cleaved Into - Big Gastrin ELISA kit
Gastrin-34 ELISA kit
G34 - Gastrin ELISA kit
Gast ELISA kit
Gas ELISA kit
Specificity This kit recognizes GT in samples. No significant cross-reactivity or interference between GT and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 96-107 102
EDTA plasma(n=8) 96-108 102
Cell culture media(n=8) 85-97 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 188.30 392.10 1452.70 185.20 368.80 1344.20
Standard deviation 10.50 17.60 69.70 9.60 15.90 57.80
CV (%) 5.58 4.49 4.80 5.18 4.31 4.30
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance