Rat Fgf23 (Fibroblast growth factor 23) Sandwich ELISA (STJE0006566)

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STJE0006566

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This Fgf23 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Fgf23
Gene ID: 170583
Uniprot ID: FGF23_RAT
Specificity: This kit recognizes Rat FGF23 in samples. No significant cross-reactivity or interference between Rat FGF23 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated at Thr-171 and Thr-178 by GALNT3 and glycosylation of Thr-178 requires previous glycosylation at Thr171. Glycosylation is necessary for secretion.it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. Phosphorylation at Ser-180 mediated by FAM20C slows down glycosylation at Thr-178 notably.
Function Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Regulator of vitamin-D metabolism. Negatively regulates osteoblasts differentiation and matrix mineralization. Acts directly on the parathyroid to decrease PTH secretion. Up-regulates EGR1 expression in the presence of KL.
Protein Name Fibroblast Growth Factor 23
Fgf-23
Database Links Reactome: R-RNO-109704
Reactome: -RNO-1257604
Reactome: -RNO-190322
Reactome: -RNO-190372
Reactome: -RNO-190373
Reactome: -RNO-190374
Reactome: -RNO-190375
Reactome: -RNO-381426
Reactome: -RNO-5654219
Reactome: -RNO-5654221
Reactome: -RNO-5654227
Reactome: -RNO-5654228
Reactome: -RNO-5654687
Reactome: -RNO-5654688
Reactome: -RNO-5654689
Reactome: -RNO-5654693
Reactome: -RNO-5654695
Reactome: -RNO-5654699
Reactome: -RNO-5654700
Reactome: -RNO-5654704
Reactome: -RNO-5654706
Reactome: -RNO-5654710
Reactome: -RNO-5654712
Reactome: -RNO-5654719
Reactome: -RNO-5654720
Reactome: -RNO-5654726
Reactome: -RNO-5654727
Reactome: -RNO-5654732
Reactome: -RNO-5654733
Reactome: -RNO-5658623
Reactome: -RNO-5673001
Reactome: -RNO-6811558
Reactome: -RNO-8957275
Cellular Localisation Secreted
Secretion Is Dependent On O-Glycosylation
Alternative ELISA Names Fibroblast Growth Factor 23 ELISA kit
Fgf-23 ELISA kit
Fgf23 ELISA kit
Specificity This kit recognizes Rat FGF23 in samples. No significant cross-reactivity or interference between Rat FGF23 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-105 102
EDTA plasma (n=8) 96-111 104
Cell culture media (n=8) 89-95 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 190.98 446.20 1851.75 192.40 482.24 1710.53
Standard deviation 10.92 20.57 98.70 10.04 21.89 82.79
CV (%) 5.72 4.61 5.33 5.22 4.54 4.84
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance