| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat F9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
F9 |
| Gene ID: |
24946 |
| Uniprot ID: |
FA9_RAT |
| Specificity: |
This kit recognizes Rat F9 in samples. No significant cross-reactivity or interference between Rat F9 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Activated by factor XIa, which excises the activation peptide. The propeptide can also be removed by snake venom protease. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. Predominantly O-glucosylated at Ser-92 by POGLUT1 in vitro. |
| Function | Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. |
| Protein Name | Coagulation Factor IxChristmas Factor Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain |
| Database Links | Reactome: R-RNO-140834Reactome: -RNO-140837Reactome: -RNO-159740Reactome: -RNO-159763Reactome: -RNO-159782 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Coagulation Factor Ix CLIA kitChristmas Factor Cleaved Into - Coagulation Factor Ixa Light Chain - Coagulation Factor Ixa Heavy Chain CLIA kitF9 CLIA kitCf9 CLIA kit |
| Specificity | This kit recognizes Rat F9 in samples. No significant cross-reactivity or interference between Rat F9 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-105 | 99 |
| EDTA plasma(n=8) | 92-105 | 98 |
| Cell culture media(n=8) | 99-113 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 103.84 | 268.99 | 676.79 | 110.53 | 262.54 | 697.90 |
| Standard deviation | 12.69 | 28.49 | 56.85 | 11.04 | 30.56 | 47.81 |
| CV (%) | 12.22 | 10.59 | 8.40 | 9.99 | 11.64 | 6.85 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
