• Rat Esm1 (Endothelial cell-specific molecule 1) Sandwich ELISA (STJE0006435)

Rat Esm1 (Endothelial cell-specific molecule 1) Sandwich ELISA (STJE0006435)

SKU:
STJE0006435

Shipping:
Free Shipping
Current Stock:
Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 46.88pg/mL
Detection Limit: 78.13~5000pg/mL
Short Description: This Esm1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Esm1
Gene ID: 64536
Uniprot ID: ESM1_RAT
Specificity: This kit recognizes Rat ESM1 in samples. No significant cross-reactivity or interference between Rat ESM1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications O-glycosylated.contains chondroitin sulfate and dermatan sulfate.
Function Involved in angiogenesis.promotes angiogenic sprouting. May have potent implications in lung endothelial cell-leukocyte interactions.
Protein Name Endothelial Cell-Specific Molecule 1
Esm-1
Pg25
Cellular Localisation Secreted
Alternative ELISA Names Endothelial Cell-Specific Molecule 1 ELISA kit
Esm-1 ELISA kit
Pg25 ELISA kit
Esm1 ELISA kit
Specificity This kit recognizes Rat ESM1 in samples. No significant cross-reactivity or interference between Rat ESM1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-97 91
EDTA plasma (n=8) 88-103 94
Cell culture media (n=8) 87-100 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 263.47 569.17 1831.44 250.91 585.07 1930.93
Standard deviation 16.49 23.56 65.38 12.60 23.58 105.62
CV (%) 6.26 4.14 3.57 5.02 4.03 5.47
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance