• Rat EPOR (Erythropoietin Receptor) Sandwich ELISA Kit (STJE0008677)

Rat EPOR (Erythropoietin Receptor) Sandwich ELISA Kit (STJE0008677)

SKU:
STJE0008677

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Applications: ELISA
Reactivity: Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity: 5.8pg/mL
Detection Limit: 15.6-1000pg/mL
Short Description: This EPOR Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time: 4.5 hrs
Gene Symbol: EporUniprot ID=EPOR_RAT"
Sample Type: serum, plasma, tissue homogenates or other biological fluids.
Tissue Specificity Isoform EPOR-F and isoform EPOR-S are expressed in bone marrow, spleen and eythroleukemia cell lines.
Post Translational Modifications On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-453 is required for PTPN6 interaction, Tyr-425 for PTPN11. Tyr-425 is also required for SOCS3 binding, but Tyr-453/Tyr-455 motif is the preferred binding site. Ubiquitinated by NOSIP.appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells. Ubiquitination at Lys-280 mediates receptor internalization, whereas ubiquitination at Lys-452 promotes trafficking of activated receptors to the lysosomes for degradation.
Function Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate LYN tyrosine kinase.
Protein Name Erythropoietin Receptor
Epo-R
Database Links
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Erythropoietin Receptor ELISA kit
Epo-R ELISA kit
Epor ELISA kit
output

Information sourced from Uniprot.org

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