• Rat Epo (Erythropoietin) Sandwich ELISA (STJE0005436)
  • Rat Epo (Erythropoietin) Sandwich ELISA (STJE0005436)

Rat Epo (Erythropoietin) Sandwich ELISA (STJE0005436)

SKU:
STJE0005436

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This Epo Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Epo
Gene ID: 24335
Uniprot ID: EPO_RAT
Specificity: This kit recognizes Rat EPO in samples. No significant cross-reactivity or interference between Rat EPO and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
Protein Name Erythropoietin
Database Links Reactome: R-RNO-9027276
Reactome: -RNO-9027284
Cellular Localisation Secreted
Alternative ELISA Names Erythropoietin ELISA kit
Epo ELISA kit
Specificity This kit recognizes Rat EPO in samples. No significant cross-reactivity or interference between Rat EPO and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 95
EDTA plasma (n=8) 95-111 101
Cell culture media (n=8) 88-103 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 221.70 514.30 1746.70 219.90 557.70 1706.10
Standard deviation 11.30 30.90 76.90 14.70 25.70 83.60
CV (%) 5.10 6.01 4.40 6.68 4.61 4.90
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance