| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19ng/mL |
| Detection Limit: |
0.31~20ng/mL |
| Short Description: |
This Eno2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Eno2 |
| Gene ID: |
24334 |
| Uniprot ID: |
ENOG_RAT |
| Specificity: |
This kit recognizes Rat NSE in samples. No significant cross-reactivity or interference between Rat NSE and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. |
| Protein Name | Gamma-Enolase2-Phospho-D-Glycerate Hydro-LyaseEnolase 2Neural EnolaseNeuron-Specific EnolaseNse |
| Database Links | Reactome: R-RNO-70171Reactome: -RNO-70263 |
| Cellular Localisation | CytoplasmCell MembraneCan Translocate To The Plasma Membrane In Either The Homodimeric (Alpha/Alpha) Or Heterodimeric (Alpha/Gamma) Form |
| Alternative ELISA Names | Gamma-Enolase ELISA kit2-Phospho-D-Glycerate Hydro-Lyase ELISA kitEnolase 2 ELISA kitNeural Enolase ELISA kitNeuron-Specific Enolase ELISA kitNse ELISA kitEno2 ELISA kitEno-2 ELISA kit |
| Specificity | This kit recognizes Rat NSE in samples. No significant cross-reactivity or interference between Rat NSE and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-108 | 100 |
| EDTA plasma (n=8) | 91-105 | 97 |
| Cell culture media (n=8) | 91-105 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 1.10 | 2.20 | 9.10 | 1.20 | 2.30 | 10.00 |
| Standard deviation | 0.10 | 0.10 | 0.30 | 0.10 | 0.10 | 0.40 |
| CV (%) | 9.09 | 4.55 | 3.30 | 8.33 | 4.35 | 4.00 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
