| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88pg/mL |
| Detection Limit: |
3.13~200pg/mL |
| Short Description: |
This rat EGR1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Egr1 |
| Gene ID: |
24330 |
| Uniprot ID: |
EGR1_RAT |
| Specificity: |
This kit recognizes Rat EGR1 in samples. No significant cross-reactivity or interference between Rat EGR1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Detected in kidney thick ascending limbs and collecting ducts (at protein level). |
| Function | Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia.regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary. Regulates the amplitude of the expression rhythms of clock genes: BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene BMAL1 in the suprachiasmatic nucleus (SCN). Regulates biosynthesis of glucocorticoid receptor GR/NR3C1 in the hippocampus and thereby may play a role in the behavioral and hypothalamic-pituitary-adrenal responses to stress in offspring. |
| Protein Name | Early Growth Response Protein 1Egr-1Nerve Growth Factor-Induced Protein ANgfi-ATranscription Factor Zif268Zinc Finger Protein Krox-24 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative CLIA Names | Early Growth Response Protein 1 CLIA kitEgr-1 CLIA kitNerve Growth Factor-Induced Protein A CLIA kitNgfi-A CLIA kitTranscription Factor Zif268 CLIA kitZinc Finger Protein Krox-24 CLIA kitEgr1 CLIA kit |
| Specificity | This kit recognizes Rat EGR1 in samples. No significant cross-reactivity or interference between Rat EGR1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 93-104 | 98 |
| EDTA plasma(n=8) | 98-111 | 103 |
| Cell culture media(n=8) | 92-105 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 10.66 | 27.53 | 70.52 | 11.32 | 28.35 | 74.40 |
| Standard deviation | 1.28 | 3.10 | 5.12 | 1.29 | 3.19 | 5.93 |
| CV (%) | 12.01 | 11.26 | 7.26 | 11.40 | 11.25 | 7.97 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
