| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.221ng/mL |
| Detection Limit: |
0.625-40ng/mL |
| Short Description: |
This EGFR2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Erbb2Uniprot ID=ERBB2_RAT" |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
serum, plasma or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1250. Dephosphorylated by PTPN12. |
| Function | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Interacts (preferentially with the tyrosine phosphorylated form) with CPNE3.this interaction occurs at the cell membrane and is increased in a growth factor heregulin-dependent manner. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A.the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth. |
| Protein Name | Receptor Tyrosine-Protein Kinase Erbb-2Epidermal Growth Factor Receptor-Related ProteinProto-Oncogene NeuProto-Oncogene C-Erbb-2P185erbb2P185neuCd Antigen Cd340 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell ProjectionRuffle MembraneEarly EndosomeCytoplasmPerinuclear RegionNucleusTranslocation To The Nucleus Requires EndocytosisProbably Endosomal Sorting And Is Mediated By Importin Beta-1/Kpnb1Also Detected In Endosome-To-Tgn Retrograde VesiclesInternalized From The Cell Membrane In Response To Egf Stimulation |
| Alternative ELISA Names | Receptor Tyrosine-Protein Kinase Erbb-2 ELISA kitEpidermal Growth Factor Receptor-Related Protein ELISA kitProto-Oncogene Neu ELISA kitProto-Oncogene C-Erbb-2 ELISA kitP185erbb2 ELISA kitP185neu ELISA kitCd Antigen Cd340 ELISA kitErbb2 ELISA kitNeu ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
