| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.38ng/mL |
| Detection Limit: |
0.63~40ng/mL |
| Short Description: |
This DPP4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Dpp4 |
| Gene ID: |
25253 |
| Uniprot ID: |
DPP4_RAT |
| Specificity: |
This kit recognizes Rat DPP4 in samples. No significant cross-reactivity or interference between Rat DPP4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing. N- and O-Glycosylated. Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. |
| Function | Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. |
| Protein Name | Dipeptidyl Peptidase 4Bile Canaliculus Domain-Specific Membrane GlycoproteinDipeptidyl Peptidase IvDpp IvGp110 GlycoproteinT-Cell Activation Antigen Cd26Cd Antigen Cd26 Cleaved Into - Dipeptidyl Peptidase 4 Membrane FormDipeptidyl Peptidase Iv Membrane Form - Dipeptidyl Peptidase 4 Soluble FormDipeptidyl Peptidase Iv Soluble Form - Dipeptidyl Peptidase 4 60 Kda Soluble FormDipeptidyl Peptidase Iv 60 Kda Soluble Form |
| Database Links | Reactome: R-RNO-381771Reactome: -RNO-400511 |
| Cellular Localisation | Dipeptidyl Peptidase 4 Soluble Form: SecretedDetected In The Serum And The Seminal FluidCell MembraneSingle-Pass Type Ii Membrane ProteinApical Cell MembraneCell ProjectionInvadopodium MembraneLamellipodium MembraneCell JunctionMembrane RaftTranslocated To The Apical Membrane Through The Concerted Action Of N- And O-Glycans And Its Association With Lipid Microdomains Containing Cholesterol And SphingolipidsRedistributed To Membrane Rafts In T-Cell In An Interleukin-12-Dependent ActivationIts Interaction With Cav1 Is Necessary For Its Translocation To Membrane RaftsColocalized With Ptprc In Membrane RaftsColocalized With Fap In Invadopodia And Lamellipodia Of Migratory Activated Endothelial Cells In Collagenous MatrixColocalized With Fap On Endothelial Cells Of Capillary-Like Microvessels But Not Large Vessels Within Invasive Breast Ductal CarcinomaColocalized With Ada At The Cell Junction In Lymphocyte-Epithelial Cell AdhesionColocalized With Igf2r In Internalized Cytoplasmic Vesicles Adjacent To The Cell Surface |
| Alternative ELISA Names | Dipeptidyl Peptidase 4 ELISA kitBile Canaliculus Domain-Specific Membrane Glycoprotein ELISA kitDipeptidyl Peptidase Iv ELISA kitDpp Iv ELISA kitGp110 Glycoprotein ELISA kitT-Cell Activation Antigen Cd26 ELISA kitCd Antigen Cd26 Cleaved Into - Dipeptidyl Peptidase 4 Membrane Form ELISA kitDipeptidyl Peptidase Iv Membrane Form - Dipeptidyl Peptidase 4 Soluble Form ELISA kitDipeptidyl Peptidase Iv Soluble Form - Dipeptidyl Peptidase 4 60 Kda Soluble Form ELISA kitDipeptidyl Peptidase Iv 60 Kda Soluble Form ELISA kitDpp4 ELISA kitCd26 ELISA kit |
| Specificity | This kit recognizes Rat DPP4 in samples. No significant cross-reactivity or interference between Rat DPP4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-112 | 102 |
| EDTA plasma (n=8) | 93-106 | 99 |
| Cell culture media (n=8) | 92-106 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.17 | 5.46 | 19.19 | 2.12 | 5.05 | 19.92 |
| Standard deviation | 0.15 | 0.25 | 0.70 | 0.15 | 0.21 | 0.63 |
| CV (%) | 6.91 | 4.58 | 3.65 | 7.08 | 4.16 | 3.16 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
