• Rat Cycs (Cytochrome c, somatic) Sandwich ELISA (STJE0005435)

Rat Cycs (Cytochrome c, somatic) Sandwich ELISA (STJE0005435)

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STJE0005435

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.23ng/mL
Detection Limit: 0.39~25ng/mL
Short Description: This Cycs Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Cycs
Gene ID: 25309
Uniprot ID: CYC_RAT
Specificity: This kit recognizes Rat Cyt-C in samples. No significant cross-reactivity or interference between Rat Cyt-C and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Binds 1 heme c group covalently per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
Protein Name Cytochrome C - Somatic
Database Links Reactome: R-RNO-111457
Reactome: -RNO-111458
Reactome: -RNO-111459
Reactome: -RNO-2151201
Reactome: -RNO-3299685
Reactome: -RNO-5620971
Reactome: -RNO-5628897
Reactome: -RNO-611105
Reactome: -RNO-9627069
Reactome: -RNO-9707564
Cellular Localisation Mitochondrion Intermembrane Space
Loosely Associated With The Inner Membrane
Alternative ELISA Names Cytochrome C - Somatic ELISA kit
Cycs ELISA kit
Specificity This kit recognizes Rat Cyt-C in samples. No significant cross-reactivity or interference between Rat Cyt-C and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-105 97
EDTA plasma (n=8) 86-97 91
Cell culture media (n=8) 96-107 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.33 4.00 8.84 1.36 3.69 8.71
Standard deviation 0.08 0.16 0.28 0.09 0.17 0.29
CV (%) 6.02 4.00 3.17 6.62 4.61 3.33
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance