• Rat Csf1 (Macrophage colony-stimulating factor 1) Sandwich ELISA (STJE0005887)

Rat Csf1 (Macrophage colony-stimulating factor 1) Sandwich ELISA (STJE0005887)

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STJE0005887

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Csf1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Csf1Uniprot ID=CSF1_RAT"
Specificity: This kit recognizes Rat MCSF in samples. No significant cross-reactivity or interference between Rat MCSF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Protein Name Macrophage Colony-Stimulating Factor 1
Csf-1
Mcsf
Proteoglycan Macrophage Colony-Stimulating Factor
Pg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Processed Macrophage Colony-Stimulating Factor 1: Secreted
Extracellular Space
Alternative ELISA Names Macrophage Colony-Stimulating Factor 1 ELISA kit
Csf-1 ELISA kit
Mcsf ELISA kit
Proteoglycan Macrophage Colony-Stimulating Factor ELISA kit
Pg-M-Csf Cleaved Into - Processed Macrophage Colony-Stimulating Factor 1 - Macrophage Colony-Stimulating Factor 1 43 Kda Subunit ELISA kit
Csf1 ELISA kit
Csfm ELISA kit
Specificity This kit recognizes Rat MCSF in samples. No significant cross-reactivity or interference between Rat MCSF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 85-99 92
EDTA plasma (n=8) 88-102 94
Cell culture media (n=8) 87-99 93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 54.82 89.16 373.18 56.59 82.81 406.55
Standard deviation 3.40 3.90 12.65 3.49 4.43 16.30
CV (%) 6.20 4.37 3.39 6.17 5.35 4.01
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance