Rat Crp (C-reactive protein) Sandwich ELISA (STJE0006537)

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STJE0006537

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This Crp Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Crp
Gene ID: 25419
Uniprot ID: CRP_RAT
Specificity: This kit recognizes Rat hs-CRP in samples. No significant cross-reactivity or interference between Rat hs-CRP and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications The last two cysteines are involved either in interchain disulfide bonds or in an intrachain bond.
Function Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Protein Name C-Reactive Protein
Database Links Reactome: R-RNO-173623
Cellular Localisation Secreted
Alternative ELISA Names C-Reactive Protein ELISA kit
Crp ELISA kit
Ptx1 ELISA kit
Specificity This kit recognizes Rat hs-CRP in samples. No significant cross-reactivity or interference between Rat hs-CRP and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-97 93
EDTA plasma (n=8) 97-109 105
Cell culture media (n=8) 90-101 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 20.13 56.75 235.50 20.39 60.86 222.49
Standard deviation 1.20 2.81 12.29 1.07 2.87 9.52
CV (%) 5.96 4.95 5.22 5.25 4.72 4.28
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance