| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19pmol/mL |
| Detection Limit: |
0.31~20pmol/mL |
| Short Description: |
This Creb1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Creb1 |
| Gene ID: |
81646 |
| Uniprot ID: |
CREB1_RAT |
| Specificity: |
This kit recognizes Rat CREB1 in samples. No significant cross-reactivity or interference between Rat CREB1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling. |
| Protein Name | Cyclic Amp-Responsive Element-Binding Protein 1Creb-1Camp-Responsive Element-Binding Protein 1 |
| Database Links | Reactome: R-RNO-198693Reactome: -RNO-199920Reactome: -RNO-375165Reactome: -RNO-442742Reactome: -RNO-881907Reactome: -RNO-9031628Reactome: -RNO-9634638 |
| Cellular Localisation | Nucleus |
| Alternative ELISA Names | Cyclic Amp-Responsive Element-Binding Protein 1 ELISA kitCreb-1 ELISA kitCamp-Responsive Element-Binding Protein 1 ELISA kitCreb1 ELISA kitCreb-1 ELISA kit |
| Specificity | This kit recognizes Rat CREB1 in samples. No significant cross-reactivity or interference between Rat CREB1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-102 | 97 |
| EDTA plasma (n=8) | 92-106 | 99 |
| Cell culture media (n=8) | 97-111 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pmol/mL) | 1.08 | 1.96 | 6.81 | 1.03 | 1.82 | 7.45 |
| Standard deviation | 0.06 | 0.08 | 0.30 | 0.07 | 0.10 | 0.38 |
| CV (%) | 5.56 | 4.08 | 4.41 | 6.80 | 5.49 | 5.10 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
