• Rat CKLF (Chemokine Like Factor) CLIA Kit (STJC0001597)

Rat CKLF (Chemokine Like Factor) CLIA Kit (STJC0001597)

SKU:
STJC0001597

Shipping:
Free Shipping
Current Stock:
Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.88pg/mL
Detection Limit: 3.13~200pg/mL
Short Description: This rat CKLF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Cklf
Gene ID: 245978
Uniprot ID: CKLF_RAT
Specificity: This kit recognizes Rat CKLF in samples. No significant cross-reactivity or interference between Rat CKLF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Both isoforms have highest expression levels in testis with relatively lower expression level in liver, spleen, lung, brain and heart and barely detectable levels in skeletal muscle and kidney were barely detected. In most tissues, isoform CKLF2 has higher expression levels than isoform CKLF1.
Function May play an important role in inflammation and regeneration of skeletal muscle. Essential for embryonic development. Isoform CKLF1: Has chemotactic response in monocytes, neutrophils and lymphocytes. Binds CCR4.
Protein Name Chemokine-Like Factor
Cellular Localisation Isoform Cklf1: Secreted
Isoform Cklf2: Membrane
Multi-Pass Membrane Protein
Alternative CLIA Names Chemokine-Like Factor CLIA kit
Cklf CLIA kit
Cklf1 CLIA kit
Specificity This kit recognizes Rat CKLF in samples. No significant cross-reactivity or interference between Rat CKLF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 97-112 103
EDTA plasma(n=8) 85-97 91
Cell culture media(n=8) 97-115 105

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 10.64 24.55 95.61 10.37 25.74 100.76
Standard deviation 1.24 2.37 7.63 1.04 2.42 7.12
CV (%) 11.65 9.65 7.98 10.03 9.40 7.07
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance