| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88pg/mL |
| Detection Limit: |
3.13~200pg/mL |
| Short Description: |
This rat CKLF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Cklf |
| Gene ID: |
245978 |
| Uniprot ID: |
CKLF_RAT |
| Specificity: |
This kit recognizes Rat CKLF in samples. No significant cross-reactivity or interference between Rat CKLF and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Both isoforms have highest expression levels in testis with relatively lower expression level in liver, spleen, lung, brain and heart and barely detectable levels in skeletal muscle and kidney were barely detected. In most tissues, isoform CKLF2 has higher expression levels than isoform CKLF1. |
| Function | May play an important role in inflammation and regeneration of skeletal muscle. Essential for embryonic development. Isoform CKLF1: Has chemotactic response in monocytes, neutrophils and lymphocytes. Binds CCR4. |
| Protein Name | Chemokine-Like Factor |
| Cellular Localisation | Isoform Cklf1: SecretedIsoform Cklf2: MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | Chemokine-Like Factor CLIA kitCklf CLIA kitCklf1 CLIA kit |
| Specificity | This kit recognizes Rat CKLF in samples. No significant cross-reactivity or interference between Rat CKLF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 97-112 | 103 |
| EDTA plasma(n=8) | 85-97 | 91 |
| Cell culture media(n=8) | 97-115 | 105 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 10.64 | 24.55 | 95.61 | 10.37 | 25.74 | 100.76 |
| Standard deviation | 1.24 | 2.37 | 7.63 | 1.04 | 2.42 | 7.12 |
| CV (%) | 11.65 | 9.65 | 7.98 | 10.03 | 9.40 | 7.07 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
