| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat CFL1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Cfl1 |
| Gene ID: |
29271 |
| Uniprot ID: |
COF1_RAT |
| Specificity: |
This kit recognizes Rat CFL1 in samples. No significant cross-reactivity or interference between Rat CFL1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Inactivated by phosphorylation on Ser-3. Phosphorylated on Ser-3 in resting cells. Dephosphorylated by PDXP/chronophin.this restores its activity in promoting actin filament depolymerization. The phosphorylation of Ser-24 may prevent recognition of the nuclear localization signal. Phosphorylated via a ARRB1-RAC1-LIMK1-PAK1 cascade upon active ligand stimulation of atypical chemokine receptor ACKR2. |
| Function | Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Important for normal progress through mitosis and normal cytokinesis. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration. |
| Protein Name | Cofilin-1Cofilin - Non-Muscle Isoform |
| Cellular Localisation | Nucleus MatrixCytoplasmCytoskeletonCell ProjectionRuffle MembranePeripheral Membrane ProteinCytoplasmic SideLamellipodium MembraneLamellipodiumGrowth ConeAxonColocalizes With The Actin Cytoskeleton In Membrane Ruffles And LamellipodiaDetected At The Cleavage Furrow And Contractile Ring During CytokinesisAlmost Completely In Nucleus In Cells Exposed To Heat Shock Or 10% Dimethyl Sulfoxide |
| Alternative CLIA Names | Cofilin-1 CLIA kitCofilin - Non-Muscle Isoform CLIA kitCfl1 CLIA kit |
| Specificity | This kit recognizes Rat CFL1 in samples. No significant cross-reactivity or interference between Rat CFL1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 91-107 | 99 |
| EDTA plasma(n=8) | 94-110 | 100 |
| Cell culture media(n=8) | 91-105 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 97.38 | 244.85 | 988.68 | 101.98 | 253.71 | 1026.98 |
| Standard deviation | 8.20 | 17.97 | 72.67 | 12.43 | 22.76 | 67.06 |
| CV (%) | 8.42 | 7.34 | 7.35 | 12.19 | 8.97 | 6.53 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
