| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Cdh1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Cdh1 |
| Gene ID: |
83502 |
| Uniprot ID: |
CADH1_RAT |
| Specificity: |
This kit recognizes Rat E-Cad in samples. No significant cross-reactivity or interference between Rat E-Cad and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. |
| Protein Name | Cadherin-1Epithelial CadherinE-CadherinUvomorulinCd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 |
| Database Links | Reactome: R-RNO-1474228Reactome: -RNO-216083Reactome: -RNO-351906Reactome: -RNO-418990Reactome: -RNO-5626467 |
| Cellular Localisation | Cell JunctionAdherens JunctionCell MembraneSingle-Pass Type I Membrane ProteinEndosomeGolgi ApparatusTrans-Golgi NetworkColocalizes With Dlgap5 At Sites Of Cell-Cell Contact In Intestinal Epithelial CellsAnchored To Actin Microfilaments Through Association With Alpha-Beta- And Gamma-CateninSequential Proteolysis Induced By Apoptosis Or Calcium InfluxResults In Translocation From Sites Of Cell-Cell Contact To The CytoplasmColocalizes With Rab11a Endosomes During Its Transport From The Golgi Apparatus To The Plasma Membrane |
| Alternative ELISA Names | Cadherin-1 ELISA kitEpithelial Cadherin ELISA kitE-Cadherin ELISA kitUvomorulin ELISA kitCd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 ELISA kitCdh1 ELISA kit |
| Specificity | This kit recognizes Rat E-Cad in samples. No significant cross-reactivity or interference between Rat E-Cad and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 96-107 | 102 |
| EDTA plasma (n=8) | 87-101 | 94 |
| Cell culture media (n=8) | 92-103 | 98 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.50 | 1.30 | 4.80 | 0.50 | 1.40 | 5.10 |
| Standard deviation | 0.03 | 0.07 | 0.23 | 0.03 | 0.08 | 0.24 |
| CV (%) | 6.00 | 5.38 | 4.79 | 6.00 | 5.71 | 4.71 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
