| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat CCKAR kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Cckar |
| Gene ID: |
24889 |
| Uniprot ID: |
CCKAR_RAT |
| Specificity: |
This kit recognizes Rat CCKAR in samples. No significant cross-reactivity or interference between Rat CCKAR and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Pancreas and brain. Also expressed in the gastrointestinal system and vagus nerve. |
| Function | Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. |
| Protein Name | Cholecystokinin Receptor Type ACck-A ReceptorCck-ArCholecystokinin-1 ReceptorCck1-R |
| Database Links | Reactome: R-RNO-375276Reactome: -RNO-416476 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | Cholecystokinin Receptor Type A CLIA kitCck-A Receptor CLIA kitCck-Ar CLIA kitCholecystokinin-1 Receptor CLIA kitCck1-R CLIA kitCckar CLIA kit |
| Specificity | This kit recognizes Rat CCKAR in samples. No significant cross-reactivity or interference between Rat CCKAR and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 98-110 | 104 |
| EDTA plasma(n=8) | 97-110 | 103 |
| Cell culture media(n=8) | 94-106 | 100 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 92.19 | 226.96 | 829.93 | 90.35 | 218.98 | 791.15 |
| Standard deviation | 9.70 | 16.66 | 79.18 | 11.18 | 17.01 | 56.57 |
| CV (%) | 10.52 | 7.34 | 9.54 | 12.37 | 7.77 | 7.15 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
