• Rat CASP3 (Caspase 3) CLIA Kit (STJC0001585)

Rat CASP3 (Caspase 3) CLIA Kit (STJC0001585)

SKU:
STJC0001585

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This rat CASP3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Casp3
Gene ID: 25402
Uniprot ID: CASP3_RAT
Specificity: This kit recognizes Rat CASP3 in samples. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in heart, brain, liver, and muscle but not in kidney or testis.
Post Translational Modifications Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface.
Protein Name Caspase-3
Casp-3
Apopain
Cysteine Protease Cpp32
Cpp-32
Irp
Lice
Protein Yama
Srebp Cleavage Activity 1
Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12
Database Links Reactome: R-RNO-111459
Reactome: -RNO-111465
Reactome: -RNO-111469
Reactome: -RNO-140342
Reactome: -RNO-2028269
Reactome: -RNO-205025
Reactome: -RNO-264870
Reactome: -RNO-351906
Reactome: -RNO-418889
Reactome: -RNO-449836
Reactome: -RNO-5620971
Cellular Localisation Cytoplasm
Alternative CLIA Names Caspase-3 CLIA kit
Casp-3 CLIA kit
Apopain CLIA kit
Cysteine Protease Cpp32 CLIA kit
Cpp-32 CLIA kit
Irp CLIA kit
Lice CLIA kit
Protein Yama CLIA kit
Srebp Cleavage Activity 1 CLIA kit
Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 CLIA kit
Casp3 CLIA kit
Cpp32 CLIA kit
Specificity This kit recognizes Rat CASP3 in samples. No significant cross-reactivity or interference between Rat CASP3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-99 93
EDTA plasma(n=8) 100-114 108
Cell culture media(n=8) 86-99 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 183.33 540.16 1769.87 198.15 506.44 1937.95
Standard deviation 21.34 57.91 160.35 17.89 51.40 122.09
CV (%) 11.64 10.72 9.06 9.03 10.15 6.30
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance