| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This Casp1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Casp1Uniprot ID=CASP1_RAT" |
| Specificity: |
This kit recognizes Rat CASP1 in samples. No significant cross-reactivity or interference between Rat CASP1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Thiol protease involved in a variety of inflammatory processes by proteolytically cleaving other proteins, such as the precursors of the inflammatory cytokines interleukin-1 beta (IL1B) and interleukin 18 (IL18) as well as the pyroptosis inducer Gasdermin-D (GSDMD), into active mature peptides. Plays a key role in cell immunity as an inflammatory response initiator: once activated through formation of an inflammasome complex, it initiates a pro-inflammatory response through the cleavage of the two inflammatory cytokines IL1B and IL18, releasing the mature cytokines which are involved in a variety of inflammatory processes. Cleaves a tetrapeptide after an Asp residue at position P1. Also initiates pyroptosis, a programmed lytic cell death pathway, through cleavage of GSDMD. In contrast to cleavage of interleukins IL1B and IL1B, recognition and cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP1 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly. |
| Protein Name | Caspase-1Casp-1Interleukin-1 Beta ConvertaseIl-1bcInterleukin-1 Beta-Converting EnzymeIceIl-1 Beta-Converting EnzymeP45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 |
| Cellular Localisation | CytoplasmCell Membrane |
| Alternative ELISA Names | Caspase-1 ELISA kitCasp-1 ELISA kitInterleukin-1 Beta Convertase ELISA kitIl-1bc ELISA kitInterleukin-1 Beta-Converting Enzyme ELISA kitIce ELISA kitIl-1 Beta-Converting Enzyme ELISA kitP45 Cleaved Into - Caspase-1 Subunit P20 - Caspase-1 Subunit P10 ELISA kitCasp1 ELISA kitIl1bc ELISA kitIl1bce ELISA kit |
| Specificity | This kit recognizes Rat CASP1 in samples. No significant cross-reactivity or interference between Rat CASP1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-103 | 95 |
| EDTA plasma (n=8) | 84-99 | 91 |
| Cell culture media (n=8) | 85-98 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 193.18 | 599.81 | 1601.24 | 191.62 | 566.55 | 1738.77 |
| Standard deviation | 13.14 | 27.35 | 61.49 | 10.77 | 31.78 | 65.73 |
| CV (%) | 6.80 | 4.56 | 3.84 | 5.62 | 5.61 | 3.78 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
