Rat C5a (Complement Component 5a) Sandwich ELISA (STJE0006546)

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STJE0006546

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Applications: ELISA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38ng/mL
Detection Limit: 15.63~1000ng/mL
Short Description: This C5a Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Specificity: This kit recognizes Rat C5a in samples. No significant cross-reactivity or interference between Rat C5a and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Specificity This kit recognizes Rat C5a in samples. No significant cross-reactivity or interference between Rat C5a and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-103 97
EDTA plasma (n=8) 92-104 99
Cell culture media (n=8) 87-97 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 44.08 113.30 497.15 45.05 120.13 443.23
Standard deviation 2.46 5.35 26.40 2.58 5.93 22.69
CV (%) 5.58 4.72 5.31 5.73 4.94 5.12
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance