| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This C4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
C4 |
| Gene ID: |
24233 |
| Uniprot ID: |
CO4_RAT |
| Specificity: |
This kit recognizes Rat C4 in samples. No significant cross-reactivity or interference between Rat C4 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Protein Name | Complement C4 Cleaved Into - Complement C4 Beta Chain - Complement C4 Alpha Chain - C4a Anaphylatoxin - Complement C4 Gamma Chain |
| Database Links | Reactome: R-RNO-166663Reactome: -RNO-174577Reactome: -RNO-381426Reactome: -RNO-8957275Reactome: -RNO-977606 |
| Cellular Localisation | SecretedSynapseCell ProjectionAxonDendrite |
| Alternative ELISA Names | Complement C4 Cleaved Into - Complement C4 Beta Chain - Complement C4 Alpha Chain - C4a Anaphylatoxin - Complement C4 Gamma Chain ELISA kitC4 ELISA kitC4a ELISA kit |
| Specificity | This kit recognizes Rat C4 in samples. No significant cross-reactivity or interference between Rat C4 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-109 | 100 |
| EDTA plasma (n=8) | 94-107 | 101 |
| Cell culture media (n=8) | 86-99 | 91 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.49 | 19.29 | 75.42 | 10.97 | 17.67 | 77.38 |
| Standard deviation | 0.58 | 0.92 | 3.93 | 0.65 | 0.84 | 3.75 |
| CV (%) | 5.53 | 4.77 | 5.21 | 5.93 | 4.75 | 4.85 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Mouse C4b (Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain]) Sandwich ELISA (STJE0005336)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/141127/308645/STJE0005336_1__67197.1648490980.png?c=1 "Mouse C4b (Complement C4-B [Cleaved into: Complement C4 beta chain; Complement C4 alpha chain; C4a anaphylatoxin; Complement C4 gamma chain]) Sandwich ELISA (STJE0005336)")
