| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
4.69ng/mL |
| Detection Limit: |
7.81~500ng/mL |
| Short Description: |
This C3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
C3 |
| Gene ID: |
24232 |
| Uniprot ID: |
CO3_RAT |
| Specificity: |
This kit recognizes Rat C3 in samples. No significant cross-reactivity or interference between Rat C3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. In chronic inflammation, acts as a chemoattractant for neutrophils. C3-beta-c: Acts as a chemoattractant for neutrophils in chronic inflammation. Acylation stimulating protein: Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2. |
| Protein Name | Complement C3 Cleaved Into - Complement C3 Beta Chain - C3-Beta-CC3bcNeutrophil Chemotactic Factor-2Encf-2 - Complement C3 Alpha Chain - C3a AnaphylatoxinNeutrophil Chemotactic Factor-1Encf-1 - Acylation Stimulating ProteinAspC3adesarg - Complement C3b Alpha' Chain - Complement C3c Alpha' Chain Fragment 1 - Complement C3dg Fragment - Complement C3g Fragment - Complement C3d Fragment - Complement C3f Fragment - Complement C3c Alpha' Chain Fragment 2 |
| Database Links | Reactome: R-RNO-173736Reactome: -RNO-174577Reactome: -RNO-198933Reactome: -RNO-375276Reactome: -RNO-381426Reactome: -RNO-418594Reactome: -RNO-6798695Reactome: -RNO-8957275Reactome: -RNO-977606 |
| Cellular Localisation | Secreted |
| Alternative ELISA Names | Complement C3 Cleaved Into - Complement C3 Beta Chain - C3-Beta-C ELISA kitC3bc ELISA kitNeutrophil Chemotactic Factor-2 ELISA kitEncf-2 - Complement C3 Alpha Chain - C3a Anaphylatoxin ELISA kitNeutrophil Chemotactic Factor-1 ELISA kitEncf-1 - Acylation Stimulating Protein ELISA kitAsp ELISA kitC3adesarg - Complement C3b Alpha' Chain - Complement C3c Alpha' Chain Fragment 1 - Complement C3dg Fragment - Complement C3g Fragment - Complement C3d Fragment - Complement C3f Fragment - Complement C3c Alpha' Chain Fragment 2 ELISA kitC3 ELISA kit |
| Specificity | This kit recognizes Rat C3 in samples. No significant cross-reactivity or interference between Rat C3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-102 | 94 |
| EDTA plasma (n=8) | 88-101 | 93 |
| Cell culture media (n=8) | 89-104 | 96 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 25.32 | 72.35 | 248.74 | 26.86 | 77.94 | 256.58 |
| Standard deviation | 1.54 | 4.27 | 8.28 | 1.85 | 4.21 | 10.37 |
| CV (%) | 6.08 | 5.90 | 3.33 | 6.89 | 5.40 | 4.04 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
