| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This rat BSP kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
IbspUniprot ID=SIAL_RAT" |
| Specificity: |
This kit recognizes Rat IBSP in samples. No significant cross-reactivity or interference between Rat IBSP and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated.glycans consist of sialylated and core-fucosylated bi-, tri- and tetraantennary chains. Sulfated on either Tyr-316 or Tyr-317. |
| Function | Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. |
| Protein Name | Bone Sialoprotein 2Bone Sialoprotein IiBsp IiCell-Binding SialoproteinIntegrin-Binding Sialoprotein |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Bone Sialoprotein 2 CLIA kitBone Sialoprotein Ii CLIA kitBsp Ii CLIA kitCell-Binding Sialoprotein CLIA kitIntegrin-Binding Sialoprotein CLIA kitIbsp CLIA kit |
| Specificity | This kit recognizes Rat BSP in samples. No significant cross-reactivity or interference between Rat BSP and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-98 | 91 |
| EDTA plasma(n=8) | 100-114 | 106 |
| Cell culture media(n=8) | 87-102 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.53 | 1.04 | 3.63 | 0.51 | 1.02 | 3.28 |
| Standard deviation | 0.06 | 0.12 | 0.28 | 0.06 | 0.07 | 0.35 |
| CV (%) | 11.32 | 11.54 | 7.71 | 11.76 | 6.86 | 10.67 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
