| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This rat BMP-3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Bmp3 |
| Gene ID: |
25667 |
| Uniprot ID: |
BMP3_RAT |
| Specificity: |
This kit recognizes Rat BMP-3 in samples. No significant cross-reactivity or interference between Rat BMP-3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in embryonic calvaria and femur, and in adult aorta, costa, femur, kidney, lung, ovary, spleen and trachea. Widely expressed throughout the adult central nervous system, including most neurons and their axons. |
| Function | Negatively regulates bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentiation and ossification. |
| Protein Name | Bone Morphogenetic Protein 3Bmp-3 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Bone Morphogenetic Protein 3 CLIA kitBmp-3 CLIA kitBmp3 CLIA kitBmp-3 CLIA kit |
| Specificity | This kit recognizes Rat BMP-3 in samples. No significant cross-reactivity or interference between Rat BMP-3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 86-99 | 92 |
| EDTA plasma(n=8) | 93-108 | 100 |
| Cell culture media(n=8) | 87-103 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 46.91 | 105.05 | 412.48 | 51.00 | 100.61 | 379.79 |
| Standard deviation | 4.32 | 8.68 | 40.79 | 5.87 | 10.04 | 35.51 |
| CV (%) | 9.21 | 8.26 | 9.89 | 11.51 | 9.98 | 9.35 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
