| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.136ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This ATP4b Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
4.5 hrs |
| Gene Symbol: |
Atp4b |
| Gene ID: |
24217 |
| Uniprot ID: |
ATP4B_RAT |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Stomach. |
| Post Translational Modifications | N-glycosylation is necessary for assembly and functional expression of the pump at the plasma membrane. |
| Function | The beta subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Plays a structural and regulatory role in the assembly and membrane targeting of a functionally active pump. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation of the alpha subunit that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). Interacts with the phosphorylation domain of the alpha subunit and functions as a ratchet, stabilizing the lumenal-open E2 conformation and preventing the reverse reaction of the transport cycle. |
| Protein Name | Potassium-Transporting Atpase Subunit BetaGastric H(+/K(+ Atpase Subunit BetaProton Pump Beta Chain |
| Database Links | Reactome: R-RNO-936837 |
| Cellular Localisation | Apical Cell MembraneSingle-Pass Type Ii Membrane ProteinCell MembraneLocalized In The Apical Canalicular Membrane Of Parietal Cells |
| Alternative ELISA Names | Potassium-Transporting Atpase Subunit Beta ELISA kitGastric H(+/K(+ Atpase Subunit Beta ELISA kitProton Pump Beta Chain ELISA kitAtp4b ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
