| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.062ng/mL |
| Detection Limit: |
0.156-10ng/mL |
| Short Description: |
This ATP1a1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Atp1a1 |
| Gene ID: |
24211 |
| Uniprot ID: |
AT1A1_RAT |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | Expressed in the central nervous system, in most motor and sensory axons of the ventral and dorsal roots, as well as in the large motor neurons of the ventral horn (at protein level). |
| Post Translational Modifications | Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity. |
| Function | This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. |
| Protein Name | Sodium/Potassium-Transporting Atpase Subunit Alpha-1Na(+/K(+ Atpase Alpha-1 SubunitSodium Pump Subunit Alpha-1 |
| Database Links | Reactome: R-RNO-5578775Reactome: -RNO-936837 |
| Cellular Localisation | Basolateral Cell MembraneMulti-Pass Membrane ProteinCell MembraneSarcolemmaCell ProjectionAxonMelanosome |
| Alternative ELISA Names | Sodium/Potassium-Transporting Atpase Subunit Alpha-1 ELISA kitNa(+/K(+ Atpase Alpha-1 Subunit ELISA kitSodium Pump Subunit Alpha-1 ELISA kitAtp1a1 ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
