| Applications: |
ELISA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.1ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Aoc1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Aoc1 |
| Uniprot ID: |
AOC1_RAT |
| Specificity: |
This kit recognizes Rat DAO in samples. No significant cross-reactivity or interference between Rat DAO and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Catalyzes the oxidative deamination of primary amines to the corresponding aldehydes with the concomitant production of hydrogen peroxide and ammonia. Its preferred substrates in vitro are the diamines histamine and 1-methylhistamine and it could therefore play a role in allergic and immune responses. Has a broad specificity for diamines and can also act on cadaverine and putrescine, two products of amino acid catabolism. It could also act on polyamines, like spermidine and spermine though less efficiently, and regulate various biological processes. |
| Protein Name | Diamine Oxidase Copper-ContainingDiamine OxidaseAmiloride-Binding Protein 1Amine Oxidase Copper Domain-Containing Protein 1Histaminase |
| Cellular Localisation | SecretedExtracellular SpaceCell MembranePeripheral Membrane ProteinExtracellular Side |
| Alternative ELISA Names | Diamine Oxidase Copper-Containing ELISA kitDiamine Oxidase ELISA kitAmiloride-Binding Protein 1 ELISA kitAmine Oxidase Copper Domain-Containing Protein 1 ELISA kitHistaminase ELISA kitAoc1 ELISA kitAbp1 ELISA kit |
| Specificity | This kit recognizes Rat DAO in samples. No significant cross-reactivity or interference between Rat DAO and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 102-113 | 105 |
| EDTA plasma (n=8) | 84-96 | 92 |
| Cell culture media (n=8) | 86-100 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.46 | 1.19 | 4.05 | 0.45 | 1.02 | 4.91 |
| Standard deviation | 0.02 | 0.06 | 0.20 | 0.03 | 0.05 | 0.23 |
| CV (%) | 4.35 | 5.04 | 4.94 | 6.67 | 4.90 | 4.68 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
